Part:BBa_K2149001
HPPD from Pseudomonas putida
This part coding a 4-hydroxyphenylpyruvate dioxygenase (HPPD) taken from Pseudomonas putida. This gene is involved in the pathway production of tocotrienol (vitamin E) and it is an oxygenase that catalyzes the second reaction in the catabolism of tyrosine - the conversion of 4-hydroxyphenylpyruvate into homogentisate (HGA). The HGA produced by 4-hydroxyphenylpyruvate dioxygenase that is one of many steps in break l-tyrosine into acetoacetate and fumarate. HPPD is part of a class of oxygenase enzymes that usually utilize α-ketoglutarate and diatomic oxygen to oxygenate or oxidize a target molecule. It is part of the cycle to create energy in almost aerobic organisms, but in eukaryotes, HPPD has a very important function that regulates blood tyrosine levels. Mutations in the N-terminal region of this protein cause the disease known as hawkinsinuria and HPPD can also be linked to one of the oldest known inherited metabolic disorders known as alkaptonuria. Plants utilize this enzyme to produce the cofactors plastoquinone and tocopherol which are essential for the plant to survive, this is why there are several HPPD inhibitor herbicides that block the activity of this enzyme and cause the plant's death.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 82
Illegal PstI site found at 964 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 82
Illegal PstI site found at 964 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 82
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 82
Illegal PstI site found at 964 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 82
Illegal PstI site found at 964
Illegal NgoMIV site found at 381
Illegal NgoMIV site found at 502 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 865
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